Flow Cytometry Core Facility
flow cytometry

Biosafety

The Flow Cytometry and Cell Sorting Core Facility is a dual-level biosafety lab which utilizes Biosafety Level 1 (BSL-1) as the minimal work practices level in the primary lab space and Biosafety Level 2 (BSL-2) in the facility’s biocontainment room. Each investigator must prepare samples in their designated laboratories. Prepared samples that are brought into the facility for analysis and sorting must be clearly identified as infectious (BSL-2) or non-infectious (BSL-1) samples. All infectious samples must be stored in the refrigerator in the biocontainment room. All non-infectious samples must be stored in the refrigerator in the main lab.

Work Practices:

  • Standard BSL-1 practices must be used at all locations in the facility EXCEPT the biocontainment room.
    • Infectious samples are NOT permitted in facility locations EXCEPT the biocontainment room.
    • Use of BSL-1 personal protective equipment (gloves and lab coat) is required in facility locations EXCEPT the biocontainment room.
  • Standard BSL-2 practices must be used within the biocontainment room at all times when infectious samples are being handled.
    • Use of BSL-2 personal protective equipment (Polyethylene coated seamless-front gown with cuffs, disposable gloves, N95 fitted mask, and safety glasses) is required in the biocontainment room when working with infectious samples.
  • Surface disinfection (Lysol I.C. Quaternary Cleaner Disinfectant, 1:256 dilution) will be performed before and after each run.
  • All of the solid biological waste will be handled as biohazardous waste and placed into autoclavable biohazard bags for autoclaving and disposal.

Failure to follow these guidelines can result in facility access being revoked.