Mice provide an excellent model system in which to study the complexities of mammalian development and cell differentiation, as well as the underlying basis of human disease. While the use of mice in biomedical research goes back over 100 years, advances in manipulating genes in mice over the past 15 to 20 years have dramatically improved the use of mice in biomedical research. The Transgenic Mouse Facility was established in 1989 to provide the University of Kentucky research community with a centralized facility for the generation of transgenic mice via DNA microinjection. Since that time, this facility has generated over 1,500 genetically modified mouse lines.
The Transgenic Mouse Facility, supported by the Office of the Vice President for Research, provides state-of-the-art equipment and expertise essential for producing transgenic mice by DNA microinjection into mouse embryos. Investigators are responsible for producing transgenic constructs and purifying DNA for microinjection. Investigators must also have IACUC and IBC approval for all studies to be carried out with mice generated by the Transgenic Mouse Facility (the facility has approval for all services provided by our facility, so it is not necessary for individual investigators to obtain these approvals). We will be happy to discuss potential research projects and methodologies to generate transgenes. A number of vectors useful for the production of transgenic mice are also available through our facility. We will provide training on the isolation of tail DNA for genotyping and breeding strategies.