DLAR Recommended Mice Anesthesia

 

Ketamine-Dexmedetomidine (Dexdomitor) Cocktails

 

Mouse Cocktail #1:  Recommended for Shorter Procedures and/or Restraint

 

 

Drug Stock

Concentration

(mg/ml)

Volume Used for Cocktail

Volume of Cocktail Administered to Mouse

Dose Administered to Mouse

Ketamine

100 mg/ml

1.25 ml

          -

75 mg/kg

Dexmedetomidine

 0.5 mg/ml

  2.5 ml

          -

 1 mg/kg

Saline

Sterile, isotonic

1.25 ml

          -

 

 

Combination

 

Total

    

     5 ml

0.1 ml / 25 gm       IP

 

 

Mouse Cocktail #2:  Recommended for Longer Surgical Procedures

 

Drug Stock Concentration

Volume Used for Cocktail

Volume of Cocktail Administered to Mouse

Dose Administered to Mouse

Ketamine

100 mg/ml

1.25 ml

          -

75 mg/kg

Dexmedetomidine

 0.5 mg/ml

 2.5 ml

          -

 1 mg/kg

Ace promazine

  10 mg/ml

0.12 ml

          -

 1 mg/kg

Saline

Sterile, isotonic

1.13 ml

          -

 

 

Combination

 

Total

   

     5 ml

0.1 ml / 25 gm IP

 

 

Atipamezole (= Antisedan) Reversal Agent

 

This anesthetic reversal agent is an alpha-2 antagonist.  It is highly recommended to use with Mouse Cocktail #2.

It is used to reverse the effects of xylazine or dexmedetomidine.

 

 

It is recommended to make a 0.25 mg/ml stock solution (from 5 mg/ml solution in purchased bottle).

 

To make 0.25 mg/ml stock solution, make a 1:20 dilution:  Add 0.5 ml Atipamezole to 9.5 ml sterile water or sterile saline.

 

Mouse Dosage:  ~1-2.5 mg/kg

 

Give 0.3 ml SC or IP of 0.25 mg/ml stock solution to 30 gm mouse.

Give 0.2 ml SC or IP of 0.25 mg/ml stock solution to 20 gm mouse.