DLAR Recommended Rat Anesthesia

 

Ketamine-Dexmedetomidine (Dexdomitor) Cocktails

 

Rat Cocktail #1

 

 

Drug Stock

Concentration

(mg/ml)

Volume Used for Cocktail

Volume of Cocktail Administered to

Rat

Dose Administered to Rat

Ketamine

100 mg/ml

 0.8 ml

          -

 50 mg/kg

Dexmedetomidine

 0.5 mg/ml

 0.8 ml

          -

 0.25 mg/kg

Saline

Sterile, isotonic

 2.4 ml

          -

 

 

Combination

 

Total

    

     4 ml

0.25 ml / 100 gm IP

 

 

Rat Cocktail #2:

 

Drug Stock Concentration

Volume Used for Cocktail

Volume of Cocktail Administered to Rat

Dose Administered to Rat

Ketamine

100 mg/ml

1.2 ml

          -

75 mg/kg

Dexmedetomidine

 0.5 mg/ml

 0.8 ml

          -

 0.25 mg/kg

Saline

Sterile, isotonic

    2 ml

          -

 

 

Combination

 

Total

   

     4 ml

0.25 ml / 100 gm IP

 

 

Atipamezole (= Antisedan) Reversal Agent

 

This anesthetic reversal agent is an alpha-2 antagonist.  It is highly recommended to use with Both Rat Cocktails # 1 & 2.  Rats may sleep for 4-5 hours if reversal agent is not administered.

It is used to reverse the effects of xylazine or dexmedetomidine.

It is available as a 5 mg/ml injectable solution. 

 

It is recommended to make a 0.25 mg/ml stock solution (from 5 mg/ml solution in purchased bottle). 

 

To make 0.25 mg/ml stock solution, make a 1:20 dilution:  Add 0.5 ml Atipamezole to 9.5 ml sterile water or sterile saline.

 

Rat Dosage:  1 mg/kg

 

Give 0.6 ml SC or IP of 0.25 mg/ml stock solution per 150 gm rat.

 

Note:  SC or IP fluid support following procedure is recommended with use of these cocktails.  Less bladder tone is evident and rats urinate frequently while anesthetized.