Survey Research Center
microarray images


Preparation of RNA samples

Note:  The quality of the RNA is essential to the overall success of the GeneChip analysis. Since the most appropriate protocol for the isolation of RNA can be source material-dependent, we recommend using a protocol that has been established for the tissues and cells being used.  In the absence of an established protocol, we suggest using one of the commercially available kits designed for RNA isolation. 

Isolation of RNA from mammalian cells or tissues

When using a commercial kit, follow the manufacturer's instructions for RNA isolation.  Good quality RNA has been obtained using the kits listed below:

Total RNA preparation from cells:  QIAGEN's RNeasy Total RNA Isolation kit.

Total RNA preparation from tissues:  Invitrogen Life Technologies' TRIzol reagent.

Note:  When total RNA is isolated using TRIzol, a second RNA cleanup step using QIAGEN RNeasy Total RNA isolation kit can be used. This gives much better yield of labeled cRNA.

In general, 0.1-0.5ug total RNA (high quality)at 0.1-0.4 ug/ul concentration is needed to obtain sufficient quantity of labeled cRNA for hybridization to GeneChip arrays. 

University of Kentucky | UK Research
Updated 1.8.13 by Donna Wall

Notice: Some Web sites to which these materials provide links for the convenience of users are not managed by the University of Kentucky. The University does not review, control, or take responsibility for the contents of those sites.