AutoMACS Magnetic Sorter
Sorting on this instrument utilizes antibodies that are labeled with an iron-containing compound, which are then separated automatically from unlabeled cells with a rare earth magnet. Cells are labeled either directly or indirectly with conjugated antibody and then are placed on the magnetic sorter for automatic separation of labeled cells. Cells can also be labeled at the same time with a fluorochome-labeled antibody and subsequently run on a flow cytometer to check purity. The AutoMACS system from Miltenyi-Biotech is a powerful enrichment tool when combined with the flow cytometers when trying to purify a rare cell type. Labeled antibodies for use in the AutoMACS sorter can be purchased through the Miltenyi website: http://www.miltenyibiotec.com/en/default.aspx.