The MACSQuant VYB is a compact benchtop flow cytometer. It has 3 spatially-separated laser beams: 405 nm (blue) diode pumped solid state (DPSS) laser and 561 nm (yellow) diode laser, with detection of 10 optical parameter. This instrument performs absolute counting, multiparameter cell analysis and rare cell analysis (using an integrated Cell Enrichment Unit), and it is fully automated. It is the choice instrument for performing multiparametric analysis of fluorescent protein expressing cells. This instrument uses MACSQuantifyTMsoftware.