Proteomics Core Facility
Proteomics bar


Overview

The Proteomics Core Facility offers proteomic analysis services to the scientific community on a fee-for-service basis. These services are available to faculty, staff and students from the University of Kentucky, from other educational institutions, and to individuals from the industrial sector. Our focus is identification of proteins and peptides and characterization of protein modifications.

In addition to mass analysis of intact proteins, the Core also performs proteolytic digestions of proteins, the products of which can be further analyzed by tandem mass spectrometry (MS/MS) for peptide sequencing and interrogation of protein modifications. The Proetomics Core of the University of Kentucky has the expertise and equipment to perform sophisticated proteomic and mass spectral analysis. Please contact us to discuss your project and to determine how we can help you reach your research goal.

Services Available

  • Identification of unknown proteins from gel pieces or solutions
  • Characterization of protein post-translational modifications and identification of the modification sites. Commonly identified modifications include phosphorylation, acetylation, methylation, and ubiquitination.
  • Sample preparation including digestion of proteins in gel or solution
  • Data analysis, including database searching for protein identification and protein modifications
  • Analysis of intact proteins
  • de novo sequence analysis of peptides using special ETD fragmentation
  • Quantitative analysis using isotope-labeling and/or label-free methods
  • 2D gel electrophoresis and staining¬†(outsourced to Kendrick labs)
  • 2D DIGE (outsourced to Applied Biomics) ¬†
  • Gel imaging
  • Gel spot cutting

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