Microarray Core Facility
156 Health Science Research Building
The University of Kentucky Microarray Facility is one of the service cores supported in part by the Office of the Vice President for Research. The MicroArray Facility provides comprehensive state-of-the-art microarray services and resources for the analysis of gene expression, including Affymetrix GeneChip Technology, preparation of RNA samples, experimental design and bioinformatics support for genomic data reduction and analysis. Fee-based services include assessment of RNA quality and concentration with an Agilent 2100 Bioanalyzer and a NanoDrop 2000 spectrophotometer, as well as RNA labeling, chip hybridization, scanning and data collection. Available instrumentation includes an Affymetrix GCS 3000 7G scanner, GeneChip Fluidics Station 450, GeneChip Hybridization Oven 640 and a Statistical and Bioinformatics Software Computer Workstation. In 2011, the facility further acquired a second-generation nCounter Analysis System from NanoString Technologies for direct measurement of copies of RNA and microRNA with high sensitivity and accuracy and can be used to genotype DNA samples for copy number variations. In addition, the facility houses a high-throughput Roche LightCycler 480 Real-Time PCR System with automated MagNA Lyser for tissue processing and two MagNA Pure Compacts for nucleic acids isolation. The PCR facility offers 96- and 384-well plate capacity on a fee-based service basis.